Comparison of manual sperm analysis with computer-assisted sperm analysis: A comparative cross-sectional study

Abstract

Background: Conventional manual semen analysis is done routinely in most reproductive laboratories. Computer-assisted semen analysis (CASA), on the other hand, is more objective and timesaving counterpart to the conventional method. Different CASA software systems are available, some are fully automated. Thus, it is essential to analyze its performance and compare it with the conventional semen analysis method. Aims and Objectives: Our objective was to analyze the same semen sample by manual method using Makler chamber and using sophisticated CASA software. Our aim was to find the level agreement between the two methods. Materials and Methods: Ethical clearance was taken before the study. All the subjects were given proper instructions on how to collect complete semen sample. n = 60 diagnosed primary infertile male patients referred from the obstetrics and gynecology department. Semen of the same patient was analyzed by manual method and CASA software in accordance with the WHO guidelines. The results obtained were analyzed using Pearson’s correlation and Bland and Altman plot. Results: On statistical analysis, “r” value obtained is 0.99 which is highly statistically significant. CASA and manual methods both agree with each other as proved by Bland and Altman plot. Conclusion: Both methods can be used to screen semen samples in reproductive laboratories. Manual method is easy, feasible, requires expert analysis, and has subjective variability. CASA, on the other hand, is faster newer method with better reproducibility.