Comparison of maceration techniques for retrieval of bones

Abstract

Introduction: Learning osteology is an essential part of anatomy curriculum. However, recently, there has been a scarcity in the availability of bones for teaching in India. The present study was conducted to compare four different maceration techniques and find the most suitable method to procure bones from an embalmed cadaver. The present study was conducted on dissected human cadavers made available from the department of anatomy. Material and Methods: For the first three methods, after dissection, the cadavers were buried in the burial section of the department for 2 years. The bones were washed after excavation. In the first method, detergent was used; in the second, quick lime was used; and in the third, hydrogen peroxide was used. In the fourth method, parts were not buried. The dissected parts were defleshed manually. The bones were freed from the joint. A paste was made using baking soda and 30% hydrogen peroxide. This was applied and bones were kept covered for 24 h. All four methods were compared. Results: Based on time required to macerate, ease of soft-tissue removal and complete procurement with minimal damage, the method using 30% H2O2 was the best. For smaller specimens, the new method of applying a paste of baking soda and H2O2 was very effective. The time consumed was very less. Discussion and Conclusion: Maceration using 30% H2O2 gave the best result. New method tried is time-saving and useful for small specimens. Soft tissue was removed easily with no/minimal damage to the bone.