Comparison of live attenuated mumps vaccine prepared in rotating bottle and cell factory

Abstract

Objective To compare the cytopathic conditions, virus titers and quality indicators of live attenuated mumps vaccine prepared in 15 L rotating bottle and 40-layer cell factory. Methods Primary chicken embryo cells were cultured in 15 L rotating bottle and 40-layer cell factory, respectively. Cells were infected by the S79 mumps virus strain after 1-3 d of culture. And the viruses were harvested after 5-7 d further culture. The single harvest virus liquids harvested by 2 methods were combined seperatedly to be the vaccine bulks. These vaccine bulks were subjected to control tests. Results The mean titer of virus cultured in rotating bottle was 6.6 lg 50% cell culture infective dose (CCID50) per ml on 0 d, and 6.1 lgCCID50 per ml on 21 d. The mean titer of virus cultured in cell factory was 6.8 lgCCID50/ml on 0 d, and 6.2 lgCCID50/ml on 21 d. All the quality indexes of live attenuated mumps vaccine prepared by 2 methods met the relevant requirements. Conclusions The cytopathic conditions and virus titers of live attenuated mumps vaccine prepared in cell factory are better than those in rotating bottle. The study lays foundation for large-scale production and technological improvement of live attenuated mumps vaccine prepared in cell factory. Key words: Mumps vaccine; Cell factory; Rotating bottle