Comparison of Invivo and Invitro PGF2α Administrations on The Sperm Quality of Gembrong Goats

Abstract

Improving semen quality of goat can be done through the administration of PGF2α in vivo (injection) or in vitro (addition to diluent media). This study aimed to analyze the effectiveness of in vivo and in vitro PGF2α administration to improve the sperm quality of Gembrong goats. In this study, four Gembrong goats were used and divided into two treatment groups, namely K1 (n=2) which received an injection of 75 μg PGF2α, and K2 (n=2), which received an injection of 1.5 ml physiological NaCl. The collection of Gembrong goat semen was carried out 30 minutes after the treatment in each group. Then, the semen was examined macroscopically and microscopically. The semen collected in K2 was diluted with a diluent and mixed with 75 μg of PGF2α, whereas the semen collected in K1 was diluted without the addition of PGF2α. T-test was performed to analyze research data on the semen quality (volume, motility, concentration, viability, and abnormalities) after the in vivo treatment as well as spermatozoa motility after the in vitro treatment. The results showed that the in vivo administration of PGF2α was able to increase the concentration of spermatozoa (x 106 sperm cells/ml) in K1 vs. K2 with concentrations of 2300 ± 1343.5 vs. 1,185±431.34 (P>0.05). Meanwhile, the in vitro administration of PGF2α was able to increase pre-freezing motility in K1 and K2 with motility values (%) of 55.98±0.74 and 70.21±0.04 (P<0.05), respectively. Based on the results, the study concluded that both in vivo and in vitro administrations of PGF2α were able to improve the semen quality of Gembrong goats.