Abstract
Spodoptera frugiperda is a major pest causing a huge economical loss to many crops. In this present study, S. frugiperda larvae were collected from maize field and grown on natural and artificial diet. Steinernema carpocapsae was stored by using two different storage methods and tested on S. frugiperda larvae for mortality. Larvae grown on natural diet showed 58 -78% mortality whereas on artificial diet, 30- 65% mortality was recorded in 24 - 48hrs in glycerol and SA-AG beads were stored at 1000IJs. Haemolymph and midgut were collected from infected larvae maintained grown on natural diet for performing biochemical assays. THC showed decreased count in larvae at 250IJ concentration. Total protein level was high, infected with glycerol stored IJs. Phenoloxidase, SOD, POD, GST and CarE assays showed low activities at all IJs concentrations. LPO levels and CAT activities showed increase in beads storage and in fresh IJs at 1000IJs concentration. Cyt P450 showed similar activity when compared with fresh IJs. Results of antioxidant and detoxification assays conclude that fresh and SA-AG beads stored IJs have similar efficacy (nearly equal effect) in infecting S. frugiperda.
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