Comparison of in vitro propagation and occurrence of morphological and genetic variation in strawberry tissue culture with various plant hormone treatments

Abstract

The objective of this study was to carry out treatment of various plant hormones in order to determine morphological and genetic variation degree of tissue-cultured strawberry. The cultivar used in this experiment was ‘Goha’ and ‘Seolhyang’, the plant hormones used for experiment were benzyladenine (BA), N-(2-Chloro-4 pyridyl)-N’- phenylurea (CPPU) and thidiazuron (TDZ), and the concentrations were 0.5, 1.0, 2.0, 4.0 mg·L-1 with each hormone. The BA treatment of the proliferation efficiency of tissue- cultured strawberry ‘Goha’ and ‘Seolhyang’ was the highest. When processing BA, CPPU and TDZ, morphological variation and genetic variation happened in strawberry ‘Goha’ and ‘Seolhyang’, especially, the variations appeared highly in CPPU treatment. The genetic variation in ‘Goha’ appeared at the concentration more than BA 0.5 mg·L-1 as 1.1%, appeared at the concentration of CPPU 0.5 mg·L-1 as 15.3%, and at the concentration of TDZ 2.0 mg·L-1 as 1.2%. The genetic variation in ‘Seolhyang’ appeared at the concentration of BA 4.0 mg·L-1 as 2.3%, and at the concentration of CPPU 0.5 mg·L-1 as 14.3%. Therefore, CPPU should not be treated during strawberry tissue culture, and BA and TDZ should be treated at low concentration.