Abstract
Objectives: The aim of the study is to compare the efficiency of Ki-67 with special stains in the stroma of different grades of Oral Squamous Cell Carcinoma (OSCC) and to evaluate the influence of these changes in predicting the prognosis of these tumors.
 Materials and Methods: A total of 30 cases of different grades of Oral Squamous Cell Carcinoma and 6 cases of control were sections and stained with Picrosirus red (PR), combined Alcian blue-periodic acid Schiff reagent (AB-PAS) and Immunohistochemical (IHC) marker Ki-67.
 Results: Collagen fibre nature using PR stain and proliferative activity of malignant epithelial cells using IHC marker Ki-67 was found to be statistically significant. Mucin presence using AB-PAS was statistically insignificant.
 Conclusion: Prognosis in different grades of oral squamous cell carcinoma can be accessed by change in collagen fibres birefringence, as the tumour progresses there is change from mature to immature collagen. Ki-67 is a good proliferative marker and shows that there is positive correlation with histological grading of oral squamous cell carcinoma. Presence of acidic or neutral mucin in OSCC needed to be further studied. For assessing the prognosis in different grades of OSCC, special stains can also be used.
 Keywords: Collagen, Mucin, Squamous cell carcinoma, Ki-67.
Highlights
The oral cavity is the site where food is received and an area of body where contact with exogenous material, microorganism and harmful agents is intense
Ki-67 expression is not observed during the G0 phase and has a low expression in the G1 and S phases, it is an excellent marker of cell division.[15]
In the present study the expression of Ki-67 in well differentiated squamous cell carcinoma, was observed in peripheral areas of islands than the central areas of squamous maturation, which suggests that less differentiated cells are located in the peripheral layer whereas central cells are highly differentiated with the ability of keratinization, no expression of Ki‐67 was observed in the central cells of the tumor island. (Fig – 1 & Fig -2) This finding correlates with the study carried out by Dwivedi N et al[17] and Birajdar SS et al.[18]
Summary
The oral cavity is the site where food is received and an area of body where contact with exogenous material, microorganism and harmful agents is intense. Histopathological grading is an important factor in determining the prognosis of oral carcinoma.[6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
