Abstract
One-of the major efforts of our laboratory is the observation of hydrated whole cell mounts in the electron microscope. Efforts to date have shown superior resolution as compared to the light microscope only in the thin portions of spread cells (Parsons et al., 1972). The thicker portions suffer from severe chromatic aberration which limits contrast and resolution even at 800kV. Thus, an image mode capable of enhancing image contrast and eliminating chromatic aberration is necessary. Furthermore, it is presently unrealistic, for the thick portions of the cell, to rely on increasing the accelerating potential. Since the selection of an image mode is a complex function of the required contrast and resolution, and the specimen under observation, we have limited ourselves to a resolution of 20.0nm as an intermediate goal, and ask what image mode will maximize the contrast.
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