Comparison of human cytochrome P450 1A1-catalysed oxidation of benzo[a]pyrene in prokaryotic and eukaryotic expression systems

Marie Stiborová,Klaus Kopka,Volker M Arlt,Heinz H Schmeiser,Radek Indra,Eva Frei,Lucie Bořek-Dohalská,Michaela Moserová,Petr Hodek

doi:10.1007/s00706-017-2002-0

Abstract

Cytochrome P450 (CYP) 1A1 is the most important enzyme activating and detoxifying the human carcinogen benzo[a]pyrene (BaP). In the previous studies, we had shown that not only the canonic NADPH:CYP oxidoreductase (POR) can act as electron donor but also cytochrome b5 and its reductase, NADH:cytochrome b5 reductase. Here, we studied the role of the expression system used on the metabolites generated and the levels of DNA adducts formed by activated BaP. We used an eukaryotic and a prokaryotic cellular system (Supersomes, microsomes isolated from insect cells, and Bactosomes, a membrane fraction of Escherichia coli, each transfected with cDNA of human CYP1A1 and POR). These were reconstituted with cytochrome b5 with and without NADH:cytochrome b5 reductase. We evaluated the effectiveness of each cofactor, NADPH and NADH, to mediate BaP metabolism. We found that both systems differ in catalysing the reactions activating and detoxifying BaP. Two BaP-derived DNA adducts were generated by the CYP1A1-Supersomes, both in the presence of NADPH and NADH, whereas NADPH but not NADH was able to support this reaction in the CYP1A1-Bactosomes. Seven BaP metabolites were found in Supersomes with NADPH or NADH, whereas NADPH but not NADH was able to generate five BaP metabolites in Bactosomes. Our study demonstrates different catalytic efficiencies of CYP1A1 expressed in prokaryotic and eukaryotic cells in BaP bioactivation indicating some limitations in the use of E. coli cells for such studies.Graphical abstract

Highlights

Benzo[a]pyrene (BaP) (Fig. 1) is a polycyclic aromatic hydrocarbon (PAH), which has been classified as human carcinogen (Group 1) by the International Agency for Research on Cancer [1]
We have demonstrated that the NADH:cytochrome b5 reductase/cytochrome b5 system can even act as sole electron donor for both reduction steps in the CYP1A1-reaction cycle catalysing the oxidation of BaP [4, 5]
The present study was performed to follow up our previous work, where we showed that the NADH:cytochrome b5 reductase/cytochrome b5 system [4, 5] can act as sole electron donor in CYP1A1 catalysed BaP oxidation in Supersomes

Summary

Introduction

Benzo[a]pyrene (BaP) (Fig. 1) is a polycyclic aromatic hydrocarbon (PAH), which has been classified as human carcinogen (Group 1) by the International Agency for Research on Cancer [1]. This is described in a review by Schneiter and Toulmay [46] who emphasise that the lipid composition determines the sorting, orientation, b Fig. 6 DNA adduct formation by BaP, measured by 32P-postlabelling, activated with human recombinant CYP1A1 in Supersomes (a) [4] and Bactosomes b, c in the presence of NADPH or NADH and the effect of cytochrome b5 (cyt b5, at a molar ratio of CYP1A1:cytochrome b5 of 1:5) on this reaction.

Results

Conclusion