Abstract
The results of studies of physiological fluids by differential scanning calorimetry (DSC) for the purpose of diagnosis and monitoring of diseases are promising. Before the DSC method is accepted in medical diagnostics, it is worth experimenting with various environmental conditions at the research stage. Among other things, it is important to choose an appropriate solvent to dilute the tested biological fluids. In this work, human blood sera DSC profiles in aqueous and PBS (phosphate-buffered saline) solutions have been compared. Visibility of haptoglobin in the DSC profile of human blood serum is much better in water solution. In addition, contributions from albumin and haptoglobin are well separated in contrast to the PBS serum solutions. The peak or shoulder at about 83 °C which represents contributions from the CH3 domain of immunoglobulin IgG1 and/or transferrin is more clearly visible in PBS solution. The results show that the type of solvent is essential when interpreting the serum DSC profile.
Highlights
Differential scanning calorimetry (DSC) is an appropriate method for assessing protein thermal stability and conformational changes
DSC profiles of human blood plasma/serum show the complex endothermic transition, which represents the weighted sum of denaturation profiles of major protein components and reflects proteome modifications in variety of diseases and conditions
This work compares the thermal denaturation profiles of human blood serum samples in aqueous and PBS buffer solutions obtained under identical other experimental conditions
Summary
Differential scanning calorimetry (DSC) is an appropriate method for assessing protein thermal stability and conformational changes. Keywords DSC · Haptoglobin · Human blood serum · Protein thermal denaturation DSC profiles of human blood plasma/serum show the complex endothermic transition, which represents the weighted sum of denaturation profiles of major protein components and reflects proteome modifications in variety of diseases and conditions.
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