Abstract
Stem cells therapy has been suggested as a promising option for the treatment of acute kidney injury (AKI). This study was performed to compare the abilities of xenogenic transplantation of human adipose stromal vascular fraction (SVF) and adipose-derived mesenchymal stem cells (AdMSCs) to facilitate the recovery of renal function and structure in a rat model of ischemia/reperfusion (IR) induced AKI. SVF or AdMSCs were transplanted to the injured kidney through intra-parenchymal injection. Significantly improved renal function and reduced tubular injury were observed in SVF and AdMSCs groups. Administration of SVF or AdMSCs contributed to significantly improved cell proliferation and markedly reduced cell apoptosis in parallel with reduced microvascular rarefaction in injured kidney. IR injury resulted in higher levels of inflammatory cytokines, whereas xenogenic transplantation of SVF or AdMSCs reduced but not induced inflammatory cytokines expression. Additionally, in vitro study showed that administration of SVF or AdMSCs could also significantly promote the proliferation and survival of renal tubular epithelial cells underwent hypoxia/reoxygenation injury through secreting various growth factors. However, cell proliferation was significantly promoted in SVF group than in AdMSCs group. In conclusion, our study demonstrated that administration of SVF or AdMSCs was equally effective in attenuating acute renal IR injury.
Highlights
AdMSCs at passge 0 and 3. (B) Flow cytometric analysis of freshly isolated stromal vascular fraction (SVF) and cultured AdMSCs at passge 3
Compare with AdMSCs, SVF can be real time obtained in a sufficient quantity without in vitro culture, which can avoid the potential risk of microbial contamination during culturing procedures[11]
Uncultured SVF has been considered as more attractive cell source than cultured AdMSCs for tissue repair and regeneration, especially for the repair of acute organ injury including Acute kidney injury (AKI)
Summary
AdMSCs at passge 0 and 3. (B) Flow cytometric analysis of freshly isolated SVF and cultured AdMSCs at passge 3. Recent studies have suggested that uncultured adipose stromal vascular fraction (SVF) might be an attractive cell source for cell-based therapy on account of the real time isolation in an adequate quantity without in vitro expansion, which could avoid the risks described above[11]. In previous studies, both our team and other groups reported that autologous uncultured SVF could protect kidney from both IR and cisplatin induced acute injury[12,13,14].
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