Comparison of HPV DNA Screening using menstrual blood collected in sanitary pads versus cervical swabs in women with both single and multiple sexual partners

Abstract

Background: Persistent high-risk HPV infection is the main cause of cervical cancer. Sexually active women are at risk of HPV infection, especially if they have multiple sexual partners. To detect HPV, the HPV DNA test can be conducted using menstrual blood samples as an alternative to cervical cancer screening. Objective: This study aims to analyze the comparison of results between HPV DNA screening with menstrual blood in sanitary pads and cervical swabs in women with single and multi-partner sexual. Methods: This study was an analytical observational study with a cross-sectional design. The sample used was 44 women (21 single partners and 23 multiple sexual partner) aged >18 years, sexually active, experiencing regular menstruation. Menstrual blood samples in sanitary pads and cervical swabs were examined PCR method. Statistical tests used Mann Whitney with a significance level of p≤0.05, diagnostic test and ROC description. Results: HPV DNA was found in 47.6% (10/21) in menstrual blood and 47.8% (11/21) in cervical swabs with a significance value of p=1,000 in women with single sexual partners. HPV DNA was detected in 82.6% (19/23) in menstrual blood and 87% (20/23) in cervical swabs with a significance value of p=1,000 in women with multiple sexual partners. Menstrual blood in sanitary pads has a sensitivity value of 90.32%, specificity 92.31%, positive predictive value 96.5%, negative predictive value 80% and accuracy of 91.32% to detect HPV DNA. Conclusion: The conclusion from this analysis shows that there is concordance between HPV DNA screening with menstrual blood in sanitary pads and cervical swabs with high accuracy values