Abstract
Two analytical methods for the determination and confirmation of ochratoxin A (OTA) in blood serum, kidney and liver of pigs were compared. Sample clean-up was based on liquid–liquid phase extraction. The detection of OTA was accomplished with high performance liquid chromatography (HPLC) combined either with fluorescence detection (FL) or electro spray ionization (ESI+) tandem mass spectrometry (MS–MS). Comparative method evaluation was based on both the investigation of spiked tissue samples and naturally contaminated tissue samples (n = 270), composed of blood serum, kidney and liver samples taken from 90 individual pigs from different regions of Serbia. The analytical results are discussed in the light of the respective validation method data and the corresponding experimental protocols. In general, analytical data obtained with LC–MS–MS offered comparable results at the sub-ppb concentration range indicating that the liquid chromatography electro spray tandem mass spectrometric (LC–MS/MS) was more specific and sensitive for the analysis and confirmation of ochratoxin A than the high pressure liquid chromatography (HPLC) after methylation of OTA.
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