Comparison of heterophil functions of modern commercial and wild-type Rio Grande turkeys

Abstract

The purpose of the present study was to measure any functional differences in peripheral blood heterophils isolated from a commercial turkey line to wild-type Rio Grande turkeys. The phagocytosis of Salmonella enteritidis, oxidative burst (OXB) and degranulation (DGR) were used as parameters of heterophil functional efficiency in these studies. Blood was collected and heterophils isolated from each line of turkeys at days 4, 7, and 14 post-hatch. On days 4 and 7 post-hatch, heterophils from Rio Grande turkeys responded to phorbol A-myristate-13-acetate with significantly greater OXB activity than commercial line A. Results from the DGR assay also revealed a greater level of activity in Rio Grande heterophils when compared with heterophils from Line A turkeys. On day 14 post-hatch, heterophils from the commercial line A responded at similar or greater levels than Rio Grande turkey heterophils in the OXB and DGR assays. No differences in the phagocytosis of S. enteritidis were observed between the lines. These results indicate that the commercial Line A turkeys may be at an immunological disadvantage during the first days post-hatch when compared with their wild-type predecessors. Based on the results of these experiments, research into the differences and similarities between the innate immune response of commercial turkey lines and wild-type turkeys may illuminate areas where commercial lines can be improved to decrease losses due to disease and to decrease pathogen contamination of turkey products while preserving performance characteristics.