Comparison of hematological parameters in two different high altitudinal populations of Batrachuperus pinchonii (Amphibian: Urodela)

Abstract

Hematological parameters are key to reflect the health status of animals and their physiological adaptation to the environment. However, few studies focused on the inter- and intra-specific variations of hematological parameters in hynobiid salamanders. Here, we examined the hematological parameters of the stream salamander, Batrachuperus pinchonii, originating from two different altitudinal populations to explore their intra-specific variation. Sexual dimorphism is only present in the erythrocyte count and males have higher mean values than females. The morphometric values of erythrocyte, hemoglobin concentration, and erythrocyte count of the high altitudinal (Jiajin) population were smaller than those of the lower altitudinal (Sandaoping) population; however, a significant difference between two populations was only revealed in the case of erythrocyte length via ANOVA. The results of linear regression showed that a significant relationship was present between body condition and erythrocyte length as well as the erythrocyte length to erythrocyte width ratio. Our findings suggest that the features of hematological parameters in B. pinchonii are reflected in the size of erythrocyte, and neither in erythrocyte count nor in hemoglobin concentration. These results provide a foundation for assessing and monitoring the health status of this salamander species, and furthermore, for understanding the physiological basis of altitudinal adaptation.