Abstract
Use of a modified set of commercial soybean cultivar differentials to discriminate between physiologic races of Phytophthora sojae frequently provides intermediate results. New isolines, in which defined Rps resistance genes have been incorporated into the soybean cultivar, Harosoy, have been examined to evaluate their effectiveness as race differentials with selected field isolates of the fungus from irrigated soybean crops. A numerical method for computing the fit of results from an inoculation test to sets of expected differential reactions, or hypothetical reaction for new races, is described. Use of the new set of differentials, based on Harosoy isolines, did not improve the overall race determination of eight selected field isolates of Phytophthora sojae, whether based on erect seedlings or confined lesion development after hypocotyl inoculation. In fact, computations using the traditional differential set generally gave a closer fit to a race. However, the presence of an additional discriminating gene, Rps5, in one of the isolines would prove useful if additional races of the pathogen arise in Australian soybean crops.
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