Abstract

DNA finger printing (restriction fragment lining than in cattle with normal rumens. The length polymorphism analysis) was employed positive correlation between incidence of liver to genetically compare Fusobacterium abscesses and ruminal lesions was the basis for necrophorum isolates of liver abscesses, the hypothesis that F. necrophorum in liver ruminal wall, and ruminal contents from the abscesses is of ruminal origin. Our approach same animal. Fusobacterium necrophorum to documenting that F. necrophorum of liver isolates from liver abscesses were genetically abscesses originates in the rumen was to show identical to the corresponding isolates from the that the rumen wall and liver abscess isolates ruminal wall in eight of nine animals tested. are genetically identical and, thus, are progeny This genetic similarity between the isolates of a single cell. supports the hypothesis that F. necrophorum in liver abscesses originates from the ruminal wall.

Highlights

  • Liver abscesses occur most often in cattle fed high grain diets

  • Fusobacterium necrophorum isolates from liver abscesses were genetically identical to the corresponding isolates from the ruminal wall in eight of nine animals tested

  • This genetic similarity between the isolates supports the hypothesis that F. necrophorum in liver abscesses originates from the ruminal wall

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Summary

Summary

DNA finger printing (restriction fragment length polymorphism analysis) was employed to genetically compare Fusobacterium necrophorum isolates of liver abscesses, ruminal wall, and ruminal contents from the same animal. Fusobacterium necrophorum isolates from liver abscesses were genetically identical to the corresponding isolates from the ruminal wall in eight of nine animals tested. This genetic similarity between the isolates supports the hypothesis that F. necrophorum in liver abscesses originates from the ruminal wall

Introduction
Experimental Procedures
Results and Discussion

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