Abstract
IntroductionSolutions that cause elective cardiac arrest are constantly evolving, but the ideal compound has not yet been found. The authors compare a new cardioplegic solution with histidine-tryptophan-glutamate (Group 2) and other one with histidine-tryptophan-cetoglutarate (Group 1) in a model of isolated rat heart.ObjectiveTo quantify the fractal dimension and Shannon entropy in rat myocytes subjected to cardioplegia solution using histidine-tryptophan with glutamate in an experimental model, considering the caspase markers, IL-8 and KI-67.MethodsTwenty male Wistar rats were anesthetized and heparinized. The chest was opened, the heart was withdrawn and 40 ml/kg of cardioplegia (with histidine-tryptophan-cetoglutarate or histidine-tryptophan-glutamate solution) was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter placed in the Langendorff apparatus for 30 min with Ringer-Locke solution. Analyzes were performed for immunohistochemical caspase, IL-8 and KI-67.ResultsThe fractal dimension and Shannon entropy were not different between groups histidine-tryptophan-glutamate and histidine-tryptophan-acetoglutarate.ConclusionThe amount of information measured by Shannon entropy and the distribution thereof (given by fractal dimension) of the slices treated with histidine-tryptophan-cetoglutarate and histidine-tryptophan-glutamate were not different, showing that the histidine-tryptophan-glutamate solution is as good as histidine-tryptophan-acetoglutarate to preserve myocytes in isolated rat heart.
Highlights
Solutions that cause elective cardiac arrest are constantly evolving. but the ideal compound has not yet been found
The amount of information measured by Shannon entropy and the distribution thereof of the slices treated with histidine-tryptophan-cetoglutarate and histidine-tryptophan-glutamate were not different. showing that the histidine-tryptophan-glutamate solution is as good as histidine-tryptophan-acetoglutarate to preserve myocytes in isolated rat heart
This study aims to assess if the study solution histidine-tryptophan-glutamate (HTG) is better than HTK by fractal dimension and Shannon entropy in rat myocytes. considering the caspase markers
Summary
Solutions that cause elective cardiac arrest are constantly evolving. but the ideal compound has not yet been found. The authors compare a new cardioplegic solution with histidine-tryptophan-glutamate (Group 2) and other one with histidine-tryptophan-cetoglutarate (Group 1) in a model of isolated rat heart. Objective: To quantify the fractal dimension and Shannon entropy in rat myocytes subjected to cardioplegia solution using histidine-tryptophan with glutamate in an experimental model. The heart was withdrawn and 40 ml/kg of cardioplegia (with histidine-tryptophan-cetoglutarate or histidine-tryptophan-glutamate solution) was infused. During cardiac surgery it is usual the temporary arrest of the heart. Alternative cardioplegia containing less potassium than Melrose’ solution were successfully introduced as the solution of St. Thomas[3]. Low-volume blood cardioplegia[4.5] and solution of histidine-tryptophan-ketoglutarate (HTK)[6]. Despite these advances. the ideal cardioplegic solution has not yet been developed[7.8]
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