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Abstract
Twenty-four stone fruit trees showing typical symptoms of plum pox virus (PPV) were tested for PPV using ELISA, RT-PCR, real-time RT-PCR and RT-LAMP. The isolates were obtained from various regions in the Czech Republic. PPV was detected in all tested plants. Part of the coding region of the coat protein gene and adjacent non-coding region were sequenced. PPV strains of individual isolates were classified according to the obtained sequences. This study provides comprehensive evaluation of the sensitivity of four techniques used for PPV detection. Five PPV strains (D, M, W, Rec and An) were observed according to analyses of obtained sequences. The results showed that the degree of sensitivity for each detection technique varied across the four chosen methods, with the RT-LAMP technique providing very sensitive results.
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