Abstract
Rapid and reliable methods for fish authentication are required to protect consumers against food fraud. Matrix-assisted laser desorption ionization time of-flight mass spectrometry (MALDI-TOF MS) is known as a fast and accurate method for microorganisms. In this study, the effect of five preparation protocols for fish samples on the quality and reproducibility of spectra using the MALDI Biotyper platform were evaluated. The suitability of the protocols for the identification of high-fat Atlantic mackerel (Scomber scombrus) and low-fat rainbow trout (Oncorhynchus mykiss) was examined in dependence on different storage temperatures and levels of food processing (fresh, refrigerated, frozen, cooked and smoked). The results of the present study showed that acquisition of reproducible and high quality main spectra projections for high-fat and low-fat fishes in fresh and frozen states was only possible by sample preparation with 25% formic acid followed by chloroform–methanol defatting. MALDI-TOF MS based identification was also possible after treating samples at 99 °C for 5 min but not for smoked fish. Furthermore, log score values for identification of frozen fish remained stable even after 14 months of storage at −20 °C.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
