Comparison of Five Anaerobic Incubation Methods for Enumeration of Clostridium perfringens from Foods

Abstract

This study compared the cost, speed, convenience, and sensitivity of five anaerobic systems. Fung's double-tube (FDT) method, the minitube method (MT), the sandwiched microtiter plate (SMP) method, and the Mitsubishi AnaeroPack System were compared with the Brewer anaerobic jar for total anaerobic bacterial counts in foods. Incubation was at 37°C for 4, 8, 12, and 24 h. The results indicated that FDT, MT, SMP, and the Mitsubishi AnaeroPack System were as sensitive as the Brewer anaerobic jar for the detection and enumeration of Clostridium perfringens from food products. The FDT, MT, and SMP methods recovered higher numbers of C. perfringens compared to the Brewer anaerobic jar (P < 0.05) after 12 and 24 h incubation. The Brewer anaerobic jar was the most expensive method.