Abstract
The aim of the present study was to determine the ferulic acid (FA) content of Radix Angelicae Sinensis (AS), Danggui-Buxue-Tang (DBT) and Danggui-Sini-Tang (DST) using the same ultra performance liquid chromatography system and method. FA was eluted using an Acquity BEH C18 column (100×2.1 mm inner diameter; 1.7 μm). A mobile phase of methanol and 0.5% acetic acid was used and a flow rate of 0.3 ml/min was selected. The calibration curve exhibited a good linear regression (R2=0.9997). The inter- and intra-day precision measurements of FA ranged between 0.27 and 3.03% and the recovery ranged between 98.44 and 101.64% with relative standard deviation (RSD) values ≤4.73%. The method was reliable and simple. The results of the chromatographic analyses indicate that the FA contents of the DBT and DST decoctions were increased compared with that of AS due to the presence of other herbs.
Highlights
Radix Angelicae Sinensis (AS), otherwise known as Danggui, has been used in traditional Chinese medicine for thousands of years and was first mentioned in Shenlong Bencao Jing (200‐300 A.D., Han Dynasty) [1]
The standard curve was prepared using Empower software from the peak area of the seven concentrations determined by ultra performance liquid chromatography (UPLC)
Further dilution of the lowest concentration working solution was performed to obtain a series of standard solutions to evaluate the limit of detection (LOD) and the limit of quantity (LOQ) of ferulic acid (FA)
Summary
Radix Angelicae Sinensis (AS), otherwise known as Danggui, has been used in traditional Chinese medicine for thousands of years and was first mentioned in Shenlong Bencao Jing (200‐300 A.D., Han Dynasty) [1]. AS has been shown to have numerous roles, including regulating the immune system and as an anticancer, anti‐inflammatory and Medicinal formulae containing AS are usually administered for benefiting vital energy, nourishing blood and regulating menstruation. The DBT formula contained Radix Astragali and AS. DBT is usually administered to females for the treatment of menopausal symptoms. The formula regulates their health by invigorating vital energy and enriching the blood [10]. DST, a traditional medicinal formula, was first recorded in Shanghan Lun [11] and is used to treat a variety of conditions, including poor extremity circulation, heart failure, shock and severe watery diarrhea. The formula is used for the therapy of infectious diseases that are complicated with hemodynamic instability [12,13]
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