Abstract
The objective of this study was to compare extraction methods for the determination of phylloquinone content in legumes and nuts. Phylloquinone was quantified using high-performance liquid chromatography by post-column derivatization and fluorescence detection. The higher analytical values in legumes were obtained using direct solvent extraction. However, the higher analytical values in nuts were acquired using enzyme extraction. Phylloquinone contents of legumes and nuts ranged from 15.26 to 37.77 and 0.00 to 22.31 µg/100 g, respectively. In addition, analytical method validation parameters, including accuracy and precision, were determined to ensure the validity of the extraction procedures for phylloquinone analysis. Our study provides reliable data on phylloquinone contents in commonly consumed legumes and nuts in Korea. Reliable data regarding vitamin content in food are needed to further understand the nutritional role of vitamin K.
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