COMPARISON OF EXPRESSION OF IMMUNE RESPONSES DEPENDING ON LEVEL OF PERIPHERAL BLOOD LYMPHOCYTE NECROSIS AND APOPTOSIS IN HUMANS

Abstract

The processes of apoptosis and necrosis are the mechanisms of programmed cell death, differing from each other in the mechanisms of their implementation. The implementation of these processes within the physiological norm is of interest in assessing their influence on the activity of immune responses. Purpose. To identify the nature of the relationship between the level of lymphocyte necrosis of peripheral blood and the expression of immune system reactions in practically healthy people. Materials and methods. The study examined 77 people. Leukograms were carried out using a XS-1000i hematology analyzer Sysmex XS-500i (Japan). Determination of cell death was carried out by flow cytometry Epics XL (Beckman Coulter, USA). Determination of cytokines, immunoglobulins (Bender MedSystems, Austria) and serotonin (DRG, Germany). The results of the study were processed using the Statistica 6 application package (StatSoft, USA). Results. It is established that the increased level the necrosis of lymphocytes is combined with increase in number the apoptosis of lymphocytes, T-helpers, lymphocytes of CD95, IgA, IgE, IgG against the background of reduction of the level CD23, HLADR, cytokines (IFN-γ, IL-6) and serotonin. Necrotic death is not associated with cellular cytolysis of cytotoxic T lymphocytes and natural killer content, but is inversely related to neutrophil phagocytosis response. Conclusion. Conclusion. The results of the study show that necrotic lymphocyte death within physiological boundaries as opposed to apoptosis increases immune response activity. Necrosis and apoptosis are subject to predominantly mature B cells with receptors to IgE and proteins of the main histocompatibility complex class II.