Abstract
Raman spectra of eubacterial ribosomal 5S RNAs of Escherichia coli, Bacillus subtilis and Thermus thermophilis and of eukaryotic 5S RNAs of yeast and rat liver have been compared. The spectra show a very high and comparable regularity in the ribophosphate backbone as indicated by the ratio 1.67±0.03 for I 812 I 1100 in all samples. The 5S RNAs studied have a similar degree of stacking of the G, A and pyrimidine bases. A high percentage of base-paired U residues between 43 and 66% is indicated. Conformational alterations occurring in 5S RNAs in the presence of Mg 2+ ions between 20 and 50 ∗C are localized mainly in the region of loop II of the molecule. The implications of these results for the 5S RNA structure are discussed.
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