Abstract
Essential oil of Eucalyptus oleosa, cultivated in Iran, was obtained by hydrodistillation and supercritical (carbon dioxide) extraction methods. The effects of supercritical carbon dioxide extraction parameters such as pressure, temperature, dynamic extraction time and modifier volume on the extraction efficiency were investigated by the three-level orthogonal array design. Regarding the percentages of α-pinene and 1,8- cineole, the optimum supercritical fluid extraction (SFE) results were obtained at the following experimental conditions: pressure = 100 atm, T = 45°C, dynamic time = 25 min, and Vmodifier = 2 mL. The composition of the extracts obtained from E. oleosa using supercritical fluid extraction was compared with the essential oil obtained by hydrodistillation. The composition of the SFE products was markedly different from the corresponding hydrodistillated oil.
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