Abstract

Objective To compare high performance liquid chromatography (HPLC) and enzyme multiplied irmunoassay technique (EMIT) method in monitoring mycophenolic acid (MPA) in renal transplantation patients.Method Plasma samples were collected from 31 adult kidney transplant recipients at 0.5,1.5,4 and 9 h after administration of mycophenolate mofetil (MMF).All these patients were given MMF,tacrolimus and prednisolone for immunosuppression.Both EMIT and HPLC were employed to determine plasma MPA concentration.Method comparison was performed using Bland & Altman and Passing & Bablok analysis.Result HPLC method and EMIT method exhibited a liner range of 0.1-50.0 mg/L and 0.1-15.0 mg/L.All of MPA concentrations at each time point had no normal distribution.The MPA concentration determined by EMIT was higher than HPLC by 1.44 (-1.88-13.58 rg/L,P＜0.05).The EMIT assay showed a mean positive bias of 29.0％ in concentration,and 19.4％ in AUC0-12h by Bland & Altman plots.The Passing & Bablok regression analysis gave an equation of MPAEMTT =0.3926 + 1.1121 × MPAHPLC,and AUCEMIT =4.7911 + 1.0929 × AUCHPLC.Conclusion It should be considered that the putative therapeutic range for MPA with the EMIT assay is higher than the range using HPLC. Key words: Kidney transplantation; Mycophenolic acid; High performance liquid chromatography; Enzyme multiplied immunoassay technique

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