Abstract
Background and Aim:Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. The study aimed to compare the Fassisi® Bovine IgG (FB-IgG) immunoassay and an enzyme-linked immunosorbent assay for quantifying bovine IgG in neonatal calf serum.Materials and Methods:A total of 277 calves of 1-10 days of age were used in this study. Blood samples were obtained, and serum was extracted by centrifuging the samples at 2740× g for 5 min at 20°C. The serum was analyzed using the FB-IgG according to the manufacturer’s specifications. Serum IgG concentrations were also determined by enzyme-linked immunosorbent assay (ELISA-IgG). FPT was defined as a serum IgG concentration <10 mg/mL.Results:The mean ELISA-IgG serum concentration was 8.40 mg/mL (SD=7.02, range=0.10-47.50 mg/mL). FPT prevalence based on the ELISA measurements was 66.8%. The prevalence of partial and full FPT based on the FB-IgG was 54.5%. The ELISA-IgG and FB-IgG results were subjected to correlation and regression analysis. Overall sensitivity and specificity of the FB-IgG were 61.1% and 58.7%, respectively. A statistically significant dependence on age was identified in the results.Conclusion:Our findings suggest that the FB-IgG rapid method is less accurate and provides no other advantages over established methods.
Highlights
And adequate uptake of colostral immunoglobulins (Ig) by calves after birth is critical for disease prevention and later milk production in dairy cows [1,2]
The enzyme-linked immunosorbent assay (ELISA)-immunoglobulin G (IgG) and Fassisi® Bovine IgG (FB-IgG) results were subjected to correlation and regression analysis
The mean IgG concentration determined by ELISA-IgG was 8.40 mg/mL, and the distribution of the original values was skewed to the right
Summary
And adequate uptake of colostral immunoglobulins (Ig) by calves after birth is critical for disease prevention and later milk production in dairy cows [1,2]. If the quality of the colostrum is poor, the calves may not receive sufficient levels of Ig – a condition referred to as failure of passive transfer (FPT) [3]. Early feeding programs were developed to provide sufficient high-quality colostrum to newborn calves [1,2,4,5]. Measurement of immunoglobulin G (IgG) concentrations in calf serum play a significant role in controlling and monitoring colostrum quality and supply. Rapid tests are routinely used to estimate serum immunoglobulin G (IgG) concentrations in diagnosing a failure of passive transfer (FPT) in calves. Immunoassay and an enzyme-linked immunosorbent assay for quantifying bovine IgG in neonatal calf serum
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