Comparison of effectiveness of red beans (Phaseolus vulgaris L.) and candlenut (Aleurites moluccana (L.) Willd) as a replacement for media sabouraud dextrose agar for Candida albicans growth

Abstract

Fungi require nutrients, a source of energy and environmental conditions for their growth. Sabouraud Dextrose Agar is a general medium frequently for fungal growth. The high cost and the difficulty of multiplying SDA media have encouraged researchers to find alternative media from red beans and candlenuts that contain carbohydrates and protein which are as a source of nutrition for the growth of Candida albicans. The research aimed determine the growth of Candida albicans on red beans (Phaseolus vulgaris L.) and candlenuts (Aleurites moluccana (L.) Willd ) media and to determine the effectiveness of both media. The method of the research is experiment (completely randomized design) with 3 treatments and 9 repetitions. The average of colonies on red beans media of 181.7 CFU/ml, and candlenuts media of 113.8 CFU/ml with an average colony diameter red beans media of 1.19 mm and 1.12 mm candlenut media. Observations processed statistically using One Way Annova. The result showed a significant difference between the count and diameter of Candida albicans growth colonies on red beans (Phaseolus vulgaris L.) and candlenut (Aleurites moluccana (L.) Willd) media, followed by the Tukey test with the best media results being red beans (Phaseolus vulgaris L.) media.