Abstract
Near Infrared (800–2500 nm) spectroscopy has been extensively used in biomedical applications, as it offers rapid, in vivo, bed-side monitoring of important haemodynamic parameters, which is especially important in critical care settings. However, the choice of NIR spectrometer needs to be investigated for biomedical applications, as both the dual beam dispersive spectrophotomer and the FTNIR spectrometer have their own advantages and disadvantages. In this study, predictive analysis of lactate concentrations in whole blood were undertaken using multivariate techniques on spectra obtained from the two spectrometer types simultaneously and results were compared. Results showed significant improvement in predicting analyte concentration when analysis was performed on full range spectral data. This is in comparison to analysis of limited spectral regions or lactate signature peaks, which yielded poorer prediction models. Furthermore, for the same region, FTNIR showed 10% better predictive capability than the dual beam dispersive NIR spectrometer.
Highlights
Near Infrared Spectroscopy, a type of molecular vibrational spectroscopy, has found immense applications in healthcare monitoring because at the wavelength range of800–1200 nm, electromagnetic radiation has the ability to penetrate tissue and be retrieved in-vivo, making it an ideal non-invasive technique [1]
In the spectra collected during this study, the peaks arising from transitions of the lactate molecule were overshadowed by the overtones for water -OH bonds, and spectra obtained using both types of instruments strongly resembled typical NIR spectra of any aqueous solution [31]
These results show that using the wavelengths pertinent to lactate in the NIR spectral region, the concentration of lactate can be predicted
Summary
Near Infrared Spectroscopy, a type of molecular vibrational spectroscopy, has found immense applications in healthcare monitoring because at the wavelength range of. Throughput: A FTIR/FTNIR spectrometer has a higher throughput compared to the dual beam spectrophotometer, owing to the avoidance of the slits or optical apertures and the use of fewer mirrors in the instrument, which increases the total energy of the incident light on a sample and reduces reflection losses. This increases SNR, and is known as Jacquinot’s advantage [12]. This study will demonstrate the capabilities and differences of NIR dual beam and FTNIR spectroscopy coupled with multivariate analysis for lactate concentration determination
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
