Comparison of DNA Extraction Methods for GM Rice and GM Rice-Derived Food Products In China

Abstract

Three cetyl trimethyl ammonium bromide (CTAB) methods, an alkaline lysis method and two commercial kits from Tiangen and Tiandz were used to extract DNA from GM rice with Cry1Ab. DNA yield, purity and amplifiability from the six methods were compared. The results revealed that the DNA extraction methods affected DNA quality and quantity. The alkaline lysis method gave the highest DNA yield, while the CTAB-III method and the two kits produced DNA of high purity, integrity and amplifiability. These results suggested that the CTAB-III method and the two kits were suitable for extraction of rice DNA for polymerase chain reaction. The CTAB-III method and the two kits were used to extract DNA from rice crackers. The results revealed that the Tiandz kit gave the highest DNA quality and quantity from rice crackers with lower cost and lesser time in DNA extraction and analysis. Therefore, the Tiandz kit is suitable for the extraction of DNA from GM rice and GM rice-derived food products. Practical Applications The research yielded information about the effect of six different DNA extraction methods. The objectives of the present study were to obtain a suitable DNA extraction method for extraction of rice or rice-derived DNA for PCR. The result showed that the Tiandz kit was suitable for the extraction of DNA from GM rice and GM rice-derived food products.