Abstract
This project has provided DOE with basic information on the abundance, distribution and activities of aerobic heterotrophs in subsurface sediments from the Southeastern Coastal Plain (Savannah River Site -- SRS) and the Western Rockies Intermountain (Idaho National Engineering Laboratory -- INEL) and Columbia Plateau (Hanford Site). We have developed a new replica plating technique for determining numbers of microaerophiles and facultatively hypoaerobic bacteria in subsurface sediment samples. We have applied the technique to vadose zone samples from INEL and Hanford (Data submitted to database at Investigator`s Meeting, Chelan, WA, September 1991). The replica planting data suggest that most of the aerobic heterotrophic bacteria isolated from these boreholes grow at in wide range of oxygen concentrations from full saturation to one or to percent of saturation. We have tested several INEL and Hanford isolates for growth rate and growth yield at both low and high oxygen concentration. All isolates grew equally well at both oxygen concentrations regardless of whether they were isolated under low or high oxygen concentrations.
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