Abstract
OBJECTIVES:This study aimed to evaluate several methods to estimate glucose consumption in the male Wister rat brain as measured by PET.METHODS:Fourteen male Wistar normoglycemic rats were studied. The input function consisted of seventeen blood samples drawn manually from the femoral artery. Glucose uptake values were calculated using the input function resulting from the arterial blood samples and the tissue time-activity curve derived from the PET images. The estimated glucose consumption rate (Ki) based on the 2-tissue compartment model (2TCM) served as the standard for comparisons with the values calculated by the Patlak analysis and with the fractional uptake rate (FUR), standardized uptake value (SUV) and glucose corrected SUV (SUVglu).RESULTS:No significant difference between the standard Ki and the Patlak Ki was observed. The standard Ki was also found to have strong correlations and concordance with the Ki value estimated by the Patlak analysis. The FUR method presented an excellent correlation with the Ki value obtained by the 2TCM/Patlak analyses, in contrast to the SUV or SUVglu.CONCLUSIONS:From a methodological point of view, the present findings confirm the theoretical limitations of the cerebral SUV and SUVglu as a substitute for Ki in the estimation of glucose consumption in the brain. Our data suggest that the FUR is the surrogate to Ki.
Highlights
Positron emission tomography (PET) combined with 18Ffluorodeoxyglucose ([18F]FDG) is a powerful tool for investigating brain metabolism in vivo [1,2,3,4]
PET is a medical imaging technique that is based on the administration of labeled drugs with positron-emitting radioisotopes, where the chemical form of the radiopharmaceutical is designed to provide information on tissue biochemistry rather than anatomy
Animals The procedures described in the present work are part of a small-animal PET experiment examining the effects of anesthetics on cerebral glucose metabolism [25]
Summary
Positron emission tomography (PET) combined with 18Ffluorodeoxyglucose ([18F]FDG) is a powerful tool for investigating brain metabolism in vivo [1,2,3,4]. PET is a medical imaging technique that is based on the administration of labeled drugs with positron-emitting radioisotopes, where the chemical form of the radiopharmaceutical is designed to provide information on tissue biochemistry rather than anatomy. The images are formed by the detection of two opposing gamma rays that are produced in the annihilation process between the positron and the electron. PET provides a means to measure the local concentrations of positron emitters and to reconstruct the images of the radiopharmaceutical distribution in the brain. The images obtained from the brains of small animals through PET are often evaluated using several quantitative analysis approaches.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
