Comparison of different protein sources on the phosphorus digestibility of soybean meal for broiler chickens determined using the regression method

Abstract

A study was conducted to evaluate the P digestibility in soybean meal (SBM) using the regression method with different basal diet that varied in protein sources. The treatments were organized in a 4 × 3 factorial arrangement, where 4 semipurified diets were formulated with varying source of protein (no protein supplement, or added casein, potato protein isolate (PPI), or dried egg albumen (DEA) at 60 g/kg) and 3 dietary levels of SBM (290, 370, or 450 g/kg). The study was a randomized complete block design with 8 replicate cages of 6 birds per cage. The experimental diets were fed from d 19 to 22 posthatching, excreta samples were collected from d 20 to 22, and ileal digesta samples were collected on d 22. Data were analyzed as a 2-way ANOVA using the GLM procedure. The digestibility and total tract utilization of DM, P, Ca, and N were affected by protein source (P < 0.05). Diets with casein presented the highest (P < 0.05) digestibility of N. Increasing SBM level in all dietary protein sources reduced (P < 0.001) the digestibility and total tract utilization of DM. The regression-derived ileal digestibility of P in SBM was 57.8 (SE = 3.78), 63.2 (SE = 5.02), 58.8 (SE = 4.53), and 35.3% (SE = 5.27) for diets without protein supplement, with casein, PPI, or DEA, respectively; the corresponding P retention were 52.2 (SE = 11.09), 83.4 (SE = 14.89), 42.6 (SE = 15.88), and 51.9% (SE = 14.67). The protein source affected (P < 0.05) the slope and intercept of P digestibility in SBM. A comparison of the coefficients using confidence intervals demonstrated that the ileal digestibility of P in SBM determined in diets with DEA was lower (P < 0.05) than the other protein sources, which did not differ from one another. These results indicate that the selection of dietary protein supplements may affect P digestibility assays using the regression method.