COMPARISON OF DIFFERENT METHODS OF CENTRIFUGATION FOR OPTIMUM PREPARATION OF PLATELET RICH PLASMA (PRP)& FRAMING A CUSTOMIZED PRP PROTOCOL-AN INNOVATIVE APPROACH

Abstract

Introduction:The rationale behind the therapeutic potential of the high concentration of platelets in PRP (Platelet Rich Plasma) for intervention procedures of soft tissue injuries is often challenged by the ability of the centrifugation methods followed in extracting the high quality PRP with prompt contented quantity too.This study aims to unravel the basic science of PRP preparation involved in centrifugation protocols, spin methods, speed and time duration of centrifugation to obtain consistent therapeutic platelets yield. Materials and Methods:30 participants were subjected to intervention procedures using PRP. For therapeutic purpose 3 centrifugation protocol types are followed. Ø Protocol 1-Conventional Old protocol Ø Protocol 2-Customised New protocol Ø Protocol 3-Spin Reversal of the routine -hard spin followed by soft spin The Platelets and WBC concentration and composition in the final autologous PRP sample harvested as a result of double centrifugation method are compared for quantity and efficiency. Results:The maximum PCF-3.21 times above baseline and PRR -64.21% are through new customized spin proving its efficiency. As determined by one-way ANOVA, there is statistically significant difference between the PROTOCOL groups (1,2,3) for the variables PRP platelet count, PRP Leucocytes, PCF, PRR. A Tukey post hoc test revealed that there is statistically significant value for Protocol 2 and Protocol 3 compared to the Protocol 1 for PRP platelet count, PCF and PRR whereas for PRP Leucocytes, there was a statistically significant difference between the Protocol 2 and Protocol 3 which again implicates the necessity of spin reversal in yielding better quantity of leucocytes. Conclusion:To produce PRP samples with consistent and reproducible compositionsof platelets and leucocytes with better quality control standard through a detailed, precise and stepwise description of the manufacturing protocol has been explained in our study. Our perspective is in standardizing a safe, simple protocol that can be followed to obtain an optimal consistent platelet yield without the use of commercial kits, which has been proved statistically too.