Abstract
Abstract DNA from Burkholderia cepacia was prepared from suspensions of pure cultures and artificially contaminated waste water. The efficacy of four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol extraction, microwave treatment, heat treatment) and six commercially available kits (Puregene ®, High Pure PCR Template Preparation Kit ®, InstaGene ®, QIAamp Tissue Kit ®, DNAzol ®, Elu-Quik ®) was compared in terms of sensitivity in a subsequent PCR. The results showed that a simple and inexpensive procedure using a lysis buffer containing proteinase K was superior to all other methods tested.
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