Comparison of different anesthetic methods in the establishment of animal model of severe acute pancreatitis in rats

Abstract

Objective To explore the establishment of severe acute pancreatitis (SAP) model in rats with different anesthetic methods. Methods Sixty healthy male SD rats were randomly divided into 2 groups. The animals were anesthesized by intraperitoneal injection of chloral hydrate or inhaling ether via trachea cannula respectively. SAP model was established by retrograde injection of 5% sodium taurocholate into biliopancreatic duct through duodenal wall. The time of anesthetic induction, maintenance and recovery was monitored. The differences in some physiological indexes were compared between the two groups. Results The time of anesthetic induction, maintenance and recovery in intraperitoneal injection group was longer than in inhaling ether group [(170±9) s, (164±7) min, (143±8) min; (9±2) s, (24±4) min, (2±1) min]. The blood pH value, PO2 and respiratory rate in intraperitoneal injection group were lower than in inhaling ether group [pH: (7.29±0.04), PO2: (129.9±7.2) mmHg (1 mmHg=0.133 kPa), (68±4) breaths per minute; pH: (7.52±0.04), PO2: (231.3±8.3) mmHg, (81±3) breaths per minute]. While, the PCO2 in intraperitoneal injection group was higher than in inhaling ether group [(59.4±3.1) mmHg vs. (34.3±2.5) mmHg]. Conclusion It is better to choose the anesthetic method of inhaling ether when establishing SAP model in rats for its less influence on the some physiological indexes. Key words: Severe acute pancreatitis; Rat; Anesthesia