Abstract
AbstractRaman spectroscopy is an emerging tool for fast bacterial identification. However, Raman spectroscopy is depending on suitable preprocessing of the spectra, thereby background removal is a decisive step for conventional Raman spectroscopy. The background has to be estimated, which is challenging especially for high fluorescence backgrounds. Shifted excitation Raman difference spectroscopy (SERDS) eliminates the background through the experimental procedure and holds as promising approach for highly fluorescent samples. Bacterial Raman spectra might be especially complex because these spectra consist of a multitude of overlapping Raman bands from a large multiplicity of biomolecules and only subtitle differences between the species Raman spectra enable the bacterial identification. Here, we investigate the benefits of SERDS compared with conventional Raman spectroscopy specific for the study and identification of bacteria. The comparison utilizes spectra sets of four bacterial species measured with conventional Raman spectroscopy and SERDS and covers three processing approaches for SERDS spectra, for example, the reconstruction with a non‐negative least square algorithm.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.