Abstract
Two 23-residue oligodeoxyribonucleotides, corresponding to both strands of a DNA duplex at the O R3 site of bacteriophage λ, have been synthesized in good yields and in milligram quantities by a solid-phase phosphotriester method using two different supports, Kieselguhr-polydimethylacrylamide composite and controlled pore glass. Rapid purification was possible using high-performance liquid chromatography on radial compression ion-exchange columns. The results and utility of the two supports are compared.
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