Comparison of Compertz and Logistic Models in Estimating the Growth of Leptin-Deficient (ob/ob) Mice and Wild-Type Mice Fed an In-House Prepared High-Fat Diet

Ah Reum Son,Beob Gyun Kim,Hyunwoong Jo,Kyu Ree Park

doi:10.3844/ajavsp.2019.1.6

Ah Reum Son, Beob Gyun Kim + Show 2 more

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https://doi.org/10.3844/ajavsp.2019.1.6

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Abstract

This experiment was conducted to growth curve models for leptin-deficient (ob/ob) and wild-type mice fed a meal-form High-Fat (HF) diet prepared in-house for over 120 weeks. Two hundred and twenty-four sets of Body Weight (BW) and age data were collected from ob/ob mice and 485 sets of data were collected from wild-type mice. All animals had free access to the HF diet (34.3% energy from fat) and water. Individual BW and survival rates of mice were measured. To develop growth curves of ob/ob mice and wild-type mice fed the HF diet, Gompertz and Logistic growth models were employed. The survival rates of ob/ob mice fed the HF diet were lower than those of wild-type mice. Models for estimating growth of ob/ob mice fed the HF diet were: BW, g = 61.36 × e-2.56×e^-0.13t in Gompertz model (Akaike’s Information Criterion, AIC = 1,694); and BW, g = 60.65 × (1 + 7.04 × e-0.18t)-1 in Logistic model (AIC = 1,719). Growth models for wild-type mice fed the HF diet were: BW, g = 28.94 × e-1.75×e^-0.13t in Gompertz model (AIC = 3,171); and BW, g = 28.23 × (1 + 4.85 × e-0.25t)-1 in Logistic model (AIC = 3,198). In conclusion, Gompertz models may be more appropriate to estimate the growth of ob/ob mouse fed the diet with high-fat concentration.

Highlights

Many obese models used in obesity or obesity-related disease research are gene-specific knockout or transgenic rodents and the obese model animals are often fed HighFat (HF) diets (Woods et al, 2003; Buettner et al, 2007)
The average mature Body Weight (BW) of ob/ob mice estimated from the Gompertz and Logistic models was approximately 61.0 g (Table 2; Fig. 3 and 4)
The average mature BW of wild-type mice estimated from the models was approximately 28.6 g

Summary

Introduction

Many obese models used in obesity or obesity-related disease research are gene-specific knockout or transgenic rodents and the obese model animals are often fed HighFat (HF) diets (Woods et al, 2003; Buettner et al, 2007). In many nutrition experiments for testing dietary supplements, on the other hand, meal-form diets are preferred due to the convenience in preparing experimental diets (Niu et al, 2017; Elkahoui et al, 2018). Young obese mice mostly less than 20 weeks of age are often used in nutrition research, older mice are more appropriate for mimicking adult or elderly human obesity and have been recently used in elderly obesity research (Hunsche et al, 2016; Frasca et al, 2017). The objectives of the present work were to test a novel meal-form HF diet fed to mice and to develop growth curve models for ob/ob and wild-type mice fed a meal-form HF diet for over 120 weeks

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