Abstract
We compared fresh bee venom samples produced by Anatolian beekeepers with commercial bee venom samples based on physicochemical analyses results. Sugar content analysis was conducted using HPLC-RID, moisture content analysis was performed using a moisture analyzer and melittin, apamin and phospholipase A2 contents were analyzed via HPLC-UV. When we compared the commercial bee venom samples with the freshly collected Anatolian honey bee venom, it was determined that the apamin, melittin and phospholipase A2 contents were generally lower in the commercial bee venom samples. Additionally, there was a statistically significant difference between the groups in terms of the moisture and phospholipase A2 contents (p <0.5). When we evaluated the sugar profile analysis, other than in maltose and erlose no difference was found between the two groups. The results showed that the content quality of the fresh bee venom samples collected from Anatolian honey bees was higher than that of the commercially sold bee venom samples. This result clearly indicated that bee venom samples intended for use in apitherapy or for cosmetic purposes should be obtained fresh or kept under very good conditions.
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