Comparison of CHO cell cultivation and mAbproduction in the Allegro™ XRS 20 and conventional rocker type single-use bioreactors

Abstract

Background The Allegro XRS 20 bioreactor system is a single-use bioreactor system suitable for applications ranging from general life sciences research to seed train operations as well as small scale production at the 2 to 25 literscale. It features a 3D rectangular biocontainer that is rocked across two independent axes perpendicular to one another. These features allow for significant improvements in mixing and kLa when compared with a conventional rocking bioreactor that features a 2D “pillow” biocontainer that rocks across a single axis. The Allegro XRS 20 controller is able to control agitation of the biocontainer over a wide range of conditions, from gentle agitation required for shear sensitive cultures, to more vigorous conditions generating higher oxygen transfer rates and decreased mixing times needed for the high productivity systems seen in the biopharmaceutical industry today. Control of pH and DO is fully automated by the optical sensors supplied with the biocontainer. Gassing strategy is managed by three independent mass flow controllers and fluid additions can be made via three integrated pumps. All operations are managed through a user friendly touch screen interface.