Abstract
Wheat germ agglutinin conjugated to colloidal gold was reacted with chitin in whole cell sections of representative ascomycetous and basidiomycetous yeasts. In Saccharomyces cerevisiae chitin was concentrated throughout the bud scar region and was of negligible levels in other regions of the wall. In contrast, chitin was distributed evenly throughout the cell wall of Cryptococcus neoformans and persistent bud scars were not observed. Isolates of Malassezia spp. had obvious bud scars with chitin restricted to the collarette portion of the scar.
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