Abstract
Pseudostellariae Radix (PR) is an important traditional Chinese medicine (TCM), which is consumed commonly for its positive health effects. However, the chemical differences of PR from different cultivated fields and germplasms are still unknown. In order to comprehensively compare the chemical compositions of PR from different cultivated fields, in this study, 1H-NMR-based metabolomics coupled with high performance liquid chromatography (HPLC) were used to investigate the different metabolites in PR from five germplasms (jr, zs1, zs2, sb, and xc) cultivated in traditional fields (Jurong, Jiangsu, JSJR) and cultivated fields (Zherong, Fujian, FJZR). A total of 34 metabolites were identified based on 1H-NMR data, and fourteen of them were found to be different in PR from JSJR and FJZR. The relative contents of alanine, lactate, lysine, taurine, sucrose, tyrosine, linolenic acid, γ-aminobutyrate, and hyperoside in PR from JSJR were higher than that in PR from FJZR, while PR from FJZR contained higher levels of glutamine, raffinose, xylose, unsaturated fatty acid, and formic acid. The contents of Heterophyllin A and Heterophyllin B were higher in PR from FJZR. This study will provide the basic information for exploring the influence law of ecological environment and germplasm genetic variation on metabolite biosynthesis of PR and its quality formation mechanism.
Highlights
Pseudostellariae Radix (Taizishen in Chinese, PR) is an arid tuberous root of Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm (Caryophyllaceae)
1 H-NMR spectra signals were assigned based on comparisons with the chemical shift of authentic authentic standards, metabolites in the Biological Magnetic Resonance Data Bank (BMRB) and NMR
A total of 34 metabolites were identified based on 1 H-NMR data, and 14 of them were significantly different in PR from different cultivated fields
Summary
Pseudostellariae Radix (Taizishen in Chinese, PR) is an arid tuberous root of Pseudostellaria heterophylla (Miq.) Pax ex Pax et Hoffm (Caryophyllaceae) It is a type of staple traditional Chinese medicine (TCM) which has the functions of strengthening the spleen, replenishing Qi, moistening lungs, and producing fluids. The quality assessment of PR are mainly focused on the quantitative determination of nucleoside, polysaccharide, saponin, pseudostellarins, and heterophyllins. Metabolomics has recently emerged as an important method for modern research research on medicinal plants, and this technology can analyze the whole components in PR, which on medicinal plants, and this technology can analyze the whole components in PR, which may fully may fully reflect the immanent quality of medicinal materials. NMR spectroscopy is a key technique reflect the immanent quality of medicinal materials.
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