Abstract
ObjectiveCattle were some of the first animals domesticated by humans for the production of milk, meat, etc. Long noncoding RNA (lncRNA) is defined as longer than 200 bp in non-protein coding transcripts. lncRNA is known to function in regulating gene expression and is currently being studied in a variety of livestock including cattle. The purpose of this study is to analyze the characteristics of lncRNA according to sex in Hanwoo cattle.MethodsThis study was conducted using the skeletal muscles of 9 Hanwoo cattle include bulls, steers and cows. RNA was extracted from skeletal muscle of Hanwoo. Sequencing was conducted using Illumina HiSeq2000 and mapped to the Bovine Taurus genome. The expression levels of lncRNAs were measured by DEGseq and quantitative trait loci (QTL) data base was used to identify QTLs associated with lncRNA. The python script was used to match the nearby genesResultsIn this study, the expression patterns of transcripts of bulls, steers and cows were identified. And we identified significantly differentially expressed lncRNAs in bulls, steers and cows. In addition, characteristics of lncRNA which express differentially in muscles according to the sex of Hanwoo were identified. As a result, we found differentially expressed lncRNAs according to sex were related to shear force and body weight.ConclusionThis study was classified and characterized lncRNA which differentially expressed by sex in Hanwoo cattle. We believe that the characterization of lncRNA by sex of Hanwoo will be helpful for future studies of the physiological mechanisms of Hanwoo cattle.
Highlights
Cattle were some of the first animals to be domesticated by humans for agricultural purposes
Expression patterns of Long noncoding RNA (lncRNA) in Hanwoo cattle by sex A hierarchical clustering analysis of the transcripts of sex transcripts in muscle tissue was performed in Hanwoo cattle
We found one common lncRNA that was differentially expressed in bulls and cows
Summary
Cattle were some of the first animals to be domesticated by humans for agricultural purposes. Analysis of intracellular transcripts has revealed that 50% of the transcribed genomes were not aligned with known protein coding regions. Many of these have been proven to have protein coding potential [5]. Non-protein coding transcripts can potentially be noncoding RNA (ncRNA) [6]. Long noncoding RNA (lncRNA) is a molecule longer than 200 bp in a non-protein-coding transcript, or one longer than 2 kb with a coding potential of an amino acid sequence less than 100 bp [7]. LncRNA is a relatively new class of RNA molecules www.ajas.info
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