Abstract
The retrospective study aimed to evaluate the diagnostic value of bronchial brushing and sputum using acid fast bacilli smear, mycobacterial culture and real-time PCR in detection of pediatric pulmonary tuberculosis, sensitivity and specificity of bronchial brushing and sputum examined by the three methods were calculated and compared to each other. Data showed there were no significant difference in sensitivity between bronchial brushing and matched sputum using each method. But the specificity of real-time PCR on bronchial brushing was lower than on sputum. Compared with bronchial brushing, sputum was better specimen in detection of pediatric pulmonary tuberculosis.
Highlights
The retrospective study aimed to evaluate the diagnostic value of bronchial brushing and sputum using acid fast bacilli smear, mycobacterial culture and real-time polymerase chain reaction (PCR) in detection of pediatric pulmonary tuberculosis, sensitivity and specificity of bronchial brushing and sputum examined by the three methods were calculated and compared to each other
* Correspondence: 13789821006@163.com; wangmaoshui@gmail.com 3Department of Lab Medicine, Shandong Provincial Chest Hospital, 46# Lishan Road, Jinan 250013Shandong, PR China Full list of author information is available at the end of the article culture (Lowenstein Jensen medium) and real-time PCR (Polymerase chain reaction) (TB RT-PCR kit, DAAN, China) in 59 pediatric patients (
Mehta J et al found that smears of brush/ wash specimens from fiberoptic bronchoscopy were of comparable sensitivity to those of sputum [5]
Summary
The retrospective study aimed to evaluate the diagnostic value of bronchial brushing and sputum using acid fast bacilli smear, mycobacterial culture and real-time PCR in detection of pediatric pulmonary tuberculosis, sensitivity and specificity of bronchial brushing and sputum examined by the three methods were calculated and compared to each other. The role of bronchial brushing for diagnosing pediatric pulmonary TB was evaluated while compared with of sputum.
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