Abstract
Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 μg/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 μg/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 μg/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms.
Highlights
Helicobacter pylori infection is recognized as an important causative agent of gastroduodenal diseases, causing chronic gastritis, peptic ulcer disease, and increased risk of cancer [1]
The MeOH extracts used in this study from Alchornea glandulosa (Euphorbiaceae) [11], Alchornea triplinervia (Euphorbiaceae) [12], Anacardium humile (Anacardiaceae) [13], Byrsonima basiloba, Byrsonima intermedia (Malpighiaceae) [14], Davilla elliptica (Dilleniaceae) [15], Qualea grandiflora (Vochysiaceae) [16], Qualea parviflora (Vochysiaceae) [17], Qualea multiflora (Vochysiaceae), and Mouriri pusa (Melastomataceae) [18, 19] were the same as the used in our previous studies
It was found that a significant kinetic profile occurs following exposures of neutrophils to different concentrations of H. pylori
Summary
Helicobacter pylori infection is recognized as an important causative agent of gastroduodenal diseases, causing chronic gastritis, peptic ulcer disease, and increased risk of cancer [1]. In Brazil, the overall prevalence of H. pylori is high when compared to that of developed countries [3]. The rate of H. pylori prevalence in adults in the south of Brazil is about 63% [4], while in a poor urban community in northeastern it is 80% [5]. Infiltration of the gastric mucosa with neutrophils, macrophages, and lymphocytes is a hallmark of H. pylori infection [6]. Myeloperoxidase, an enzyme released from the azurophilic granules in neutrophils, uses H2O2 and chloride ions (Cl−) as substrates to produce hypochlorous acid (HOCl), an important
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