Comparison of bone marrow mononuclear cells derived from stroke and normal rats for stroke treatment

Abstract

Objective To compare the therapeutic effect and angiogenic capability of bone marrow mononuclear cells derived from normal rats and ischemic rats for stroke. Methods The MCAO model was established with suture emboli method; bone marrow mononuclear cells (BMMNCs) were isolated with density gradient centrifugation method.The level of vascular endothelial growth factor(VEGF), basic fibroblast growth factor(bFGF), angiopoietin-1( Ang -1) were tested by ELISA under normoxic and hypoxic conditions.The rats were injected with PBS or 1×107 BMMNCs via femoral vein at day 1 after stroke.Immunofluorescence assay was performed at day 14 after cell transplantation. Results When compared with Nor-BMMNCs(VEGF: (96.0±12.6)pg/ml, bFGF: (107.1±19.6)pg/ml, Ang-1: (103.0±17.0)pg/ml), hypoxic stimulation significantly increased the secretion (VEGF: (114.2±10.6)pg/ml, bFGF: (133.6±19.7)pg/mL, Ang-1: (131.3±21.9) pg/ml)at day 1 after cell culture(P<0.05). The neurological deficits in rats of Isch-BMMNCs group were obviously improved compared with those in rats of Nor-BMMNCs group and PBS group at day 10 after transplantation(P<0.05). In addition, Isch-BMMNCs also significantly increased the number of microvessels and decreased the infract volume((372.5±40.1)/mm2, (18.7±4.5)%) when compared with Nor-BMMNCs group((314.0±40.1)/mm2, (26.5±7.2)%) and PBS group((222.3±56.7)/mm2, (34.5±6.3)%) at day 14 in vivo (P<0.05). Conclusion Isch-BMMNCs exert more neuroprotective effects than Nor-BMMNCs, which may relate with the difference in the secretion capability of angiogenic factors of this two original cells. Key words: Ischemic stroke; Cells transplantation; BMMMNCs; Angiogenesis; Cytokines