Abstract
Bioethanol is an alternative energy source that is increasingly needed along with the depletion of petroleum stocks. Bioethanol can be produced by utilizing various wastes, including jackfruit peel and a mixture of crude cellulose enzymes from Trichoderma reesei and Aspergillus niger as well as fermentation using Saccharomyces cerevisiae and Zymomonas mobilis. This study aimed to compare the production of bioethanol using S. cerevisiae and Z. mobilis in fermented jackfruit peel with a mixture of crude cellulose enzymes from T. reesei and A. niger. The experimental design used a completely randomized design with the ratio of crude cellulase enzymes from T. reesei and A. niger as independent variables (0:0), (1:0), (0:1), (1:1), (1:2), (2:1), (1:3), and (3:1) as well as sugar and ethanol content as dependent variables. The sugar content was determined using the DNS method, while the ethanol content was determined using an alcoholmeter. Data analysis used one way ANOVA assisted by SPSS 16. The results showed that the highest sugar content (14.21 percent) was obtained in the ratio of crude cellulase enzymes T. reesei and A. niger (1:3), while the highest ethanol content (3, 16 percent) at a ratio of 1: 2 and fermented using Z. mobilis.
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