Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells.

Hanseul Oh,Su-Jung Shin,Soyeon Park,Sunil Kim,Egan Kim,Dongzi Chen,Euiseong Kim,Sukjoon Lee

doi:10.3390/ma13225242

Abstract

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

Highlights

The purpose of root canal obturation is to form a tight barrier to protect the apical tissue from various microorganisms in the oral cavity [1]
It is necessary to evaluate the biocompatibility of sealers for human periodontal ligament stem cells
Isolation and Culture of human periodontal ligament stem cells (hPDLSCs) hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages ranged from 16 to 24 years

Summary

Introduction

The purpose of root canal obturation is to form a tight barrier to protect the apical tissue from various microorganisms in the oral cavity [1]. The previous effort to form a tight barrier was to fill the root canal with gutta percha, provided that the gutta percha has better properties and biocompatibility than the sealer [1,2,3]. With the advent of calcium silicate-based sealers, the single-cone technique was recommended, and the amount of sealer entering the root canal was increased [4]. If the sealers are extruded beyond the physiological apical foramen, the unset sealers can irritate periapical tissues [6]. It is necessary to evaluate the biocompatibility of sealers for human periodontal ligament stem cells (hPDLSCs)

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Methods

Conclusion